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maackia amurensis lectin ii malii  (Vector Laboratories)


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    Vector Laboratories maackia amurensis lectin ii malii
    Maackia Amurensis Lectin Ii Malii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/B-1265-1/Biotinylated+Maackia+Amurensis+Lectin+II+(MAL+II)/pm42863691-56-6-13
    Average 96 stars, based on 414 article reviews
    maackia amurensis lectin ii malii - by Bioz Stars, 2026-11
    96/100 stars

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    Control:

    Article Title: Galectin-like protein 2: A new non-mammalian member of the galectin family.
    Article Snippet: To detect specific binding to sialic acids, control slides were incubated with α2-3,6,8 neuraminidase (New England Biolabs) overnight at 37◦C prior to the blocking step. .. The control slides and slides not pretreated with neuraminidase were then incubated overnight at 4◦C in HEPES buffer containing biotinylated Sambucus nigra lectin (SNA) or Maackia amurensis lectin II (MAL II) (both from Vector Laboratories). .. Binding of all four lectins was detected using streptavidin AF555 (MoBiTec, Hamburg, Germany) conjugate.

    Incubation:

    Article Title: Galectin-like protein 2: A new non-mammalian member of the galectin family.
    Article Snippet: To detect specific binding to sialic acids, control slides were incubated with α2-3,6,8 neuraminidase (New England Biolabs) overnight at 37◦C prior to the blocking step. .. The control slides and slides not pretreated with neuraminidase were then incubated overnight at 4◦C in HEPES buffer containing biotinylated Sambucus nigra lectin (SNA) or Maackia amurensis lectin II (MAL II) (both from Vector Laboratories). .. Binding of all four lectins was detected using streptavidin AF555 (MoBiTec, Hamburg, Germany) conjugate.

    Article Title: Generation of ST6GAL1/ST3GAL4 double-knockout Landrace pigs with reduced respiratory sialylated glycans and decreased influenza A virus susceptibility of derived fibroblasts in vitro
    Article Snippet: Sequential blocking was performed using ReadyProbe Streptavidin Blocking Solution (Cat. # R37628 , Invitrogen) and ReadyProbes Biotin Blocking Solution (Cat. # R37627 , Invitrogen), with intervening PBS washes. .. Cells and tissues were then incubated with biotinylated lectins MAL II (1∶200, Cat. #B-1265-1, Vector Laboratories, Burlingame, CA, USA) or SNA (1∶200, Cat. #B-1305-2, Vector Laboratories) for 1 h, followed by three washes with PBS containing 0.05% Tween-20 (PBST). .. Alexa Fluor 594-conjugated streptavidin (1∶2000, Cat. #35107ES60, Yeasen, Shanghai, China) was applied in the dark for 1 h, after which cells and tissues were washed with PBST and counterstained with DAPI for nuclear visualization.

    Article Title: CHO cell production of a single, enveloped VLP vaccine co-displaying SARS-CoV-2, influenza and RSV antigens
    Article Snippet: .. For lectin blotting, membranes were then physically sectioned prior to incubation, and separate sections were probed with biotinylated Maackia amurensis lectin II (MAL II [1:500; Vector Laboratories, B-1265]) to detect α2,3-linked sialoglycoproteins or biotinylated Erythrina cristagalli lectin (ECL [1:500; Vector Laboratories, B-1145-5]) to detect terminal galactose residues. .. Both were followed by incubation with HRP-conjugated streptavidin (1:2000; BD Pharmingen, 554066).



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    maackia amurensis lectin ii malii  (Vector Laboratories)
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    biotinylated lectins mal ii  (Vector Laboratories)
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    Restoration of influenza virus susceptibility in ST6GAL1 −/− / ST3GAL4 −/− Landrace pig cells through complementation. A: Quantitative reverse transcription-PCR (RT-qPCR) analysis of ST6GAL1 and ST3GAL4 gene expression levels in wild-type (WT), knockout (KO), and complemented (KO + ST) cells. B: Immunofluorescent detection of α2,6-linked and α2,3-linked sialylated glycans in wild-type (WT), knockout (KO), and complemented (KO + ST) Landrace pig cells. Red indicates SNA (α2,6) or <t>MAL</t> Ⅱ (α2,3) staining, and blue indicates nuclei. The KO group showed nearly undetectable signals for both SNA and MAL Ⅱ. In contrast, the WT and KO + ST groups exhibited strong fluorescence for both <t>lectins,</t> indicating successful restoration of both α2,6- and α2,3-linked sialylated glycans in complemented cells. Scale bars, 100 μm. C: Cytopathic effects induced by H1N1 influenza virus infection at 0, 24, and 48 h post-infection (HPI). Scale bars, 500 μm. D: Immunofluorescent detection of IAV nucleoprotein (NP) in cells post-infection with H1N1 influenza virus at 48 HPI. NP-Alexa Fluor 488, green; DAPI, blue. Scale bars, 100 μm. E: RT-qPCR analysis of influenza viral M gene expression levels. F: Plaque assay measurement of infectious IAV (H1N1) virus titers (lg PFU/mL). Data are presented as mean ± standard deviation (A, E, and F); n = 3 independent cell culture experiments conducted on separate days with identical cell batches. Statistical significance was determined by one-way ANOVA followed by Tukey's multiple comparisons test. * P < 0.05, ** P < 0.01, and *** P < 0.001.
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    biotinylated maackia amurensis lectin ii (mal ii)  (Vector Laboratories)
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    Vector Laboratories biotinylated maackia amurensis lectin ii (mal ii)
    Restoration of influenza virus susceptibility in ST6GAL1 −/− / ST3GAL4 −/− Landrace pig cells through complementation. A: Quantitative reverse transcription-PCR (RT-qPCR) analysis of ST6GAL1 and ST3GAL4 gene expression levels in wild-type (WT), knockout (KO), and complemented (KO + ST) cells. B: Immunofluorescent detection of α2,6-linked and α2,3-linked sialylated glycans in wild-type (WT), knockout (KO), and complemented (KO + ST) Landrace pig cells. Red indicates SNA (α2,6) or <t>MAL</t> Ⅱ (α2,3) staining, and blue indicates nuclei. The KO group showed nearly undetectable signals for both SNA and MAL Ⅱ. In contrast, the WT and KO + ST groups exhibited strong fluorescence for both <t>lectins,</t> indicating successful restoration of both α2,6- and α2,3-linked sialylated glycans in complemented cells. Scale bars, 100 μm. C: Cytopathic effects induced by H1N1 influenza virus infection at 0, 24, and 48 h post-infection (HPI). Scale bars, 500 μm. D: Immunofluorescent detection of IAV nucleoprotein (NP) in cells post-infection with H1N1 influenza virus at 48 HPI. NP-Alexa Fluor 488, green; DAPI, blue. Scale bars, 100 μm. E: RT-qPCR analysis of influenza viral M gene expression levels. F: Plaque assay measurement of infectious IAV (H1N1) virus titers (lg PFU/mL). Data are presented as mean ± standard deviation (A, E, and F); n = 3 independent cell culture experiments conducted on separate days with identical cell batches. Statistical significance was determined by one-way ANOVA followed by Tukey's multiple comparisons test. * P < 0.05, ** P < 0.01, and *** P < 0.001.
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    Restoration of influenza virus susceptibility in ST6GAL1 −/− / ST3GAL4 −/− Landrace pig cells through complementation. A: Quantitative reverse transcription-PCR (RT-qPCR) analysis of ST6GAL1 and ST3GAL4 gene expression levels in wild-type (WT), knockout (KO), and complemented (KO + ST) cells. B: Immunofluorescent detection of α2,6-linked and α2,3-linked sialylated glycans in wild-type (WT), knockout (KO), and complemented (KO + ST) Landrace pig cells. Red indicates SNA (α2,6) or MAL Ⅱ (α2,3) staining, and blue indicates nuclei. The KO group showed nearly undetectable signals for both SNA and MAL Ⅱ. In contrast, the WT and KO + ST groups exhibited strong fluorescence for both lectins, indicating successful restoration of both α2,6- and α2,3-linked sialylated glycans in complemented cells. Scale bars, 100 μm. C: Cytopathic effects induced by H1N1 influenza virus infection at 0, 24, and 48 h post-infection (HPI). Scale bars, 500 μm. D: Immunofluorescent detection of IAV nucleoprotein (NP) in cells post-infection with H1N1 influenza virus at 48 HPI. NP-Alexa Fluor 488, green; DAPI, blue. Scale bars, 100 μm. E: RT-qPCR analysis of influenza viral M gene expression levels. F: Plaque assay measurement of infectious IAV (H1N1) virus titers (lg PFU/mL). Data are presented as mean ± standard deviation (A, E, and F); n = 3 independent cell culture experiments conducted on separate days with identical cell batches. Statistical significance was determined by one-way ANOVA followed by Tukey's multiple comparisons test. * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Journal: Journal of Biomedical Research

    Article Title: Generation of ST6GAL1/ST3GAL4 double-knockout Landrace pigs with reduced respiratory sialylated glycans and decreased influenza A virus susceptibility of derived fibroblasts in vitro

    doi: 10.7555/JBR.39.20250300

    Figure Lengend Snippet: Restoration of influenza virus susceptibility in ST6GAL1 −/− / ST3GAL4 −/− Landrace pig cells through complementation. A: Quantitative reverse transcription-PCR (RT-qPCR) analysis of ST6GAL1 and ST3GAL4 gene expression levels in wild-type (WT), knockout (KO), and complemented (KO + ST) cells. B: Immunofluorescent detection of α2,6-linked and α2,3-linked sialylated glycans in wild-type (WT), knockout (KO), and complemented (KO + ST) Landrace pig cells. Red indicates SNA (α2,6) or MAL Ⅱ (α2,3) staining, and blue indicates nuclei. The KO group showed nearly undetectable signals for both SNA and MAL Ⅱ. In contrast, the WT and KO + ST groups exhibited strong fluorescence for both lectins, indicating successful restoration of both α2,6- and α2,3-linked sialylated glycans in complemented cells. Scale bars, 100 μm. C: Cytopathic effects induced by H1N1 influenza virus infection at 0, 24, and 48 h post-infection (HPI). Scale bars, 500 μm. D: Immunofluorescent detection of IAV nucleoprotein (NP) in cells post-infection with H1N1 influenza virus at 48 HPI. NP-Alexa Fluor 488, green; DAPI, blue. Scale bars, 100 μm. E: RT-qPCR analysis of influenza viral M gene expression levels. F: Plaque assay measurement of infectious IAV (H1N1) virus titers (lg PFU/mL). Data are presented as mean ± standard deviation (A, E, and F); n = 3 independent cell culture experiments conducted on separate days with identical cell batches. Statistical significance was determined by one-way ANOVA followed by Tukey's multiple comparisons test. * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Article Snippet: Cells and tissues were then incubated with biotinylated lectins MAL II (1∶200, Cat. #B-1265-1, Vector Laboratories, Burlingame, CA, USA) or SNA (1∶200, Cat. #B-1305-2, Vector Laboratories) for 1 h, followed by three washes with PBS containing 0.05% Tween-20 (PBST).

    Techniques: Virus, Reverse Transcription, Quantitative RT-PCR, Analysis, Gene Expression, Knock-Out, Staining, Fluorescence, Infection, Plaque Assay, Standard Deviation, Cell Culture